Flow Cytometry Group

Jim Simone
Section Leader, Flow Cytometry
Office of Science and Technology
Phone: (301) 594-6191
Fax: (301) 402-2209
E-mail: simonej@mail.nih.gov

Overview

The primary goal of the Flow Cytometry Section is to provide the services of state-of-the-art multi-parameter cellular analysis and cell sorting for researchers and investigators of the NIAMS IRP. The Section maintains and updates the equipment as needed, provides training for investigators, schedules sorting, and collaborates for creative solutions to scientific experiments. Particular interests include multi-parameter analysis, fluorescence resonance energy transfer, single cell sorting, and cytometric analysis of intracellular signaling events.

The facility currently houses the following instrumentation: For cell analysis:

  • (Two) BD Biosciences FACSVerse (2-lasers, 8-color with Universal Loader)
  • Beckman Coulter CyAn ADP (3-laser, 9-color analyzer)
  • BD Biosciences FACSCanto (3-laser, 8-color analyzer with HTS)
  • BD Biosciences LSR Fortessa (7-laser, 18-color, with HTS)

For cell sorting:

  • Beckman Coulter MoFlo Legacy (3-laser, 10-color, with CyClone)
  • BD Biosciences FACSAria IIIu (5-laser, 18-color, with ACDU)
  • BD Biosciences Influx (7-laser, 18-color, with ACDU and spectral analyzer)

To schedule training on our cell analyzers click here. After receiving training, you can schedule your cell analysis by using the Office and Science and Technology scheduling system (for NIH use only).

For cell sorting requests, please use our scheduling system (for NIH use only).


Selected Publications

Juan AH, Derfoul A, Feng X, Ryall JG, Dell'orso S, Pasut A, Zare H, Simone JM, Rudnicki MA, Sartorelli V. Polycomb EZH2 controls self-renewal and safeguards the transcriptional identity of skeletal muscle stem cells. Genes Dev. 2011 Apr 15;25(8):789-94. PubMed Icon

Kimura A, Martin C, Robinson GW, Simone JM, Chen W, Wickre MC, O'Shea JJ, Hennighausen L. The gene encoding the hematopoietic stem cell regulator CCN3/NOV is under direct cytokine control through the transcription factors STAT5A/B. J Biol Chem. 2010 Oct 22;285(43):32704-9. PubMed Icon

Kimura A, Rieger MA, Simone JM, Chen W, Wickre MC, Zhu BM, Hoppe PS, O'Shea JJ, Schroeder T, Hennighausen L. The transcription factors STAT5A/B regulate GM-CSF-mediated granulopoiesis. Blood. 2009 Nov 19;114(21):4721-8. PubMed Icon

He L, Wu X, Simone J, Hewgill D, Lipsky PE. Determination of tumor necrosis factor receptor-associated factor trimerization in living cells by CFP->YFP->mRFP FRET detected by flow cytometry. Nucleic Acids Res. 2005; 33(6):e61. PubMed Icon

He L, Wu X, Meylan F, Olson DP, Simone J, Hewgill D, Siegel R, Lipsky PE. Monitoring caspase activity in living cells using fluorescent proteins and flow cytometry. Am J Pathol. 2004; 164(6): 1901-13. PubMed Icon

See extended list of publications

 

Reviewed December 21, 2012